UV-VIS Spectrophotometer
Ultraviolet and visible (UV-Vis) absorption spectroscopy is the measurement of the attenuation of a beam of light after it passes through a sample or after reflection from a sample surface. The absorbance of a solution increases as attenuation of the beam increases. The concentration of an analyte in solution can be determined by measuring the absorbance at a specific wavelength. UV-Vis spectroscopy is usually applied to molecules or inorganic complexes in solution. Many molecules absorb ultraviolet or visible light and different molecules absorb radiation of different wavelengths. An absorption spectrum shows a number of absorption bands corresponding to structural groups within the molecule.
Instrument: Perkin Elmer Lambda 35 UV-Vis Spektrofotometre
Specifications
- Wavelength Range: 190-1100 nm.
- Bandwidth: 0.5, 1, 2, 4 nm variable.
- Sources: Deuterium and tungsten lamps.
- Optics: Double beam.
Sample Requirements
If the sample is in solution, the solvent and the sample preparation procedure used should be stated properly. The sample solution should be clear and s sufficient amount of solvent must be supplied for blank measurements.
Ultraviolet and visible (UV-Vis) absorption spectroscopy is the measurement of the attenuation of a beam of light after it passes through a sample or after reflection from a sample surface. The absorbance of a solution increases as attenuation of the beam increases. The concentration of an analyte in solution can be determined by measuring the absorbance at a specific wavelength. UV-Vis spectroscopy is usually applied to molecules or inorganic complexes in solution. Many molecules absorb ultraviolet or visible light and different molecules absorb radiation of different wavelengths. An absorption spectrum shows a number of absorption bands corresponding to structural groups within the molecule.
Instrument: Perkin Elmer Lambda 35 UV-Vis Spektrofotometre
Specifications
- Wavelength Range: 190-1100 nm.
- Bandwidth: 0.5, 1, 2, 4 nm variable.
- Sources: Deuterium and tungsten lamps.
- Optics: Double beam.
Sample Requirements
If the sample is in solution, the solvent and the sample preparation procedure used should be stated properly. The sample solution should be clear and s sufficient amount of solvent must be supplied for blank measurements.