Instrument: ABI 3900

The ABI 3900 DNA Expression Device synthesizes oligonucleotides attached to polystyrene support by combining nucleotides one by one.

Synthesis Scale Options

• 40 nmol
• 0.2 µmol
• 1.0 µmol

Specifications

• Up to 48 oligonucleotides can be synthesized simultaneously in one operation.
• 48 primers at 40 nmol and 0.2 µmol scales can be synthesized in less than 1.5 hours.

Operations supported by the ABI 3900 device

Instrument: Hetoholten model safe 2010-1.2 class II safety cabinet

Desktop, closed-safe work environment

Specifications

DNA microarrays allow the analysis of thousands of genes simultaneously. Affymetrix GeneChip DNA Microarray is used to determine the gene expression levels, gene copy numbers and SNPs in different tissues derived from various organisms. In gene expression level studies; for each mRNA molecule in the actual RNA population; labeled, single-stranded, conjugate cDNA is prepared. The probes are generally produced in the presence of labeled nucleotides by reverse transcription of mRNA to single stranded cDNA. Biotin marked cDNAs are applied onto the chip surface.

DNA microarrays allow the analysis of thousands of genes simultaneously. Affymetrix GeneChip DNA Microarray is used to determine the gene expression levels, gene copy numbers and SNPs in different tissues derived from various organisms. In gene expression level studies; for each mRNA molecule in the actual RNA population; labeled, single-stranded, conjugate cDNA is prepared. The probes are generally produced in the presence of labeled nucleotides by reverse transcription of mRNA to single stranded cDNA. Biotin marked cDNAs are applied onto the chip surface.

Instruments: Applied Biosystems GeneAmp® PCR System 9700

The GeneAmp® PCR System 9700 is a device designed for the automatic oxidation of nucleic acids by the Polymerase Chain Reaction (PCR) method.

Specifications

Instrument: Stuart Scientific, Model S102HS

The hybridization oven is used in Southern/Northern hybridization and DNA Fingerprinting studies. The system can be used with membranes and probes ready for hybridization.