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Real Time PCR

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Real-time PCR is the most widely used PCR method giving both qualitative and quantitative results. In contrast to classical PCR based on an end product analysis, Real-Time PCR Systems monitor PCR reactions simultaneously as the reaction occurs. In such systems, the amount of PCR product produced in successive cycles is proportional to the released fluorescence signal. This signal increases in proportion to the resulting PCR products generated in each reaction cycle. Amount of fluorescence signal is recorded at each cycle, and PCR reaction is monitored during its logarithmic phase.

Molecular Biology and Biotechnology R&D Center

Genome Analysis Laboratory (GEN)

Agarose Gel Electrophoresis System (DNA)
Bioanalyzer
Classic PCR Device
DNA Sequence Analysis
High Efficiency DNA Expression Device
Image Analysis System
Low-Volume Spectrophotometer
Microarray
Microarray Workstation
Microcentrifuge
Real Time PCR
UV Safety Cabinet


Laboratories

Molecular Biology and Biotechnology R&D Center

Genome Analysis Laboratory (GEN)

Agarose Gel Electrophoresis System (DNA)
Bioanalyzer
Classic PCR Device
DNA Sequence Analysis
High Efficiency DNA Expression Device
Image Analysis System
Low-Volume Spectrophotometer
Microarray
Microarray Workstation
Microcentrifuge
Real Time PCR
UV Safety Cabinet


Laboratories

Share
Tweet
  • English
  • Türkçe
Listen

Real-time PCR is the most widely used PCR method giving both qualitative and quantitative results. In contrast to classical PCR based on an end product analysis, Real-Time PCR Systems monitor PCR reactions simultaneously as the reaction occurs. In such systems, the amount of PCR product produced in successive cycles is proportional to the released fluorescence signal. This signal increases in proportion to the resulting PCR products generated in each reaction cycle. Amount of fluorescence signal is recorded at each cycle, and PCR reaction is monitored during its logarithmic phase.

Merkezi Laboratuvar ARGE Eğitim Ölçme Merkezi Üniversiteler Mahallesi, Dumlupınar Bulvarı No:1, 06800 Çankaya/Ankara © ORTA DOĞU TEKNİK ÜNİVERSİTESİ ANKARA KAMPUSU