Real-time PCR is the most widely used PCR method giving both qualitative and quantitative results. In contrast to classical PCR based on an end product analysis, Real-Time PCR Systems monitor PCR reactions simultaneously as the reaction occurs. In such systems, the amount of PCR product produced in successive cycles is proportional to the released fluorescence signal. This signal increases in proportion to the resulting PCR products generated in each reaction cycle. Amount of fluorescence signal is recorded at each cycle, and PCR reaction is monitored during its logarithmic phase.